Nitrogenaza
| Nitrogenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 | |||||||||
| Identifikatori | |||||||||
| EC broj | 1.18.6.1 | ||||||||
| CAS broj | 2598062 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Nitrogenaza (EC 1.18.6.1, Nitrogenase) je enzim sa sistematskim imenom redukovani feredoksin:dinitrogen oksidoreduktaza (ATP-hidroliza).[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 8 redukovani feredoksin + 8 H+ + N2 + 16 ATP + 16H2O 8 oksidovani feredoksin + H2 + 2 NH3 + 16 ADP + 16 fosfat
Za dejstvo ovog enzim je neohodan Mg2+. On se sastoji od dva proteina. Oni se mogu razdvojiti. Oba proteina su neophodna za nitrogenazno dejstvo.
Reference
- ↑ Zumft, W.G., Paneque, A., Aparicio, P.J. and Losada, M. (1969). „Mechanism of nitrate reduction in Chlorella”. Biochem. Biophys. Res. Commun. 36: 980-986. PMID 4390523.
- ↑ Liang, J. and Burris, R.H. (1988). „Hydrogen burst associated with nitrogenase-catalyzed reactions”. Proc. Natl. Acad. Sci. USA 85: 9446-9450. PMID 3200830.
- ↑ Dance, I. (2004). „The mechanism of nitrogenase. Computed details of the site and geometry of binding of alkyne and alkene substrates and intermediates”. J. Am. Chem. Soc. 126: 11852-11863. PMID 15382920.
- ↑ Chan, J.M., Wu, W., Dean, D.R. and Seefeldt, L.C. (2000). „Construction and characterization of a heterodimeric iron protein: defining roles for adenosine triphosphate in nitrogenase catalysis”. Biochemistry 39: 7221-7228. PMID 10852721.
Literatura
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.
Spoljašnje veze
- MeSH Nitrogenase
- p
- r
- u
Aktivno mesto • Alosterna regulacija • Mesto vezivanja • Katalitički perfektan enzim • Koenzim • Kofaktor • Kooperativnost • EC broj • Enzimska kataliza • Inhibicija enzima • Enzimska kinetika • Lajnviver–Burk dijagram • Mihaelis–Mentenova kinetika • Spisak enzima
EC1 Oksidoreduktaze/spisak • EC2 Transferaze/spisak • EC3 Hidrolaze/spisak • EC4 Lijaze/spisak • EC5 Izomeraze/spisak • EC6 Ligaze/spisak
B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, 4.1/2/3/4/5/6, 5.1/2/3/4/99, 6.1-3/4/5-6
