TaqI

Restriction enzyme

TaqI is a restriction enzyme isolated from the bacterium Thermus aquaticus in 1978.[1] It has a recognition sequence of 

5'TCGA
3'AGCT

and makes the cut 

5'---T   CGA---3'
3'---AGC   T---5'

References

  1. ^ Sato, S. (February 1978). "A single cleavage of Simian virus 40 (SV40) DNA by a site specific endonuclease from Thermus aquaticus, Taq I". J. Biochem. 83 (2): 633–5. doi:10.1093/oxfordjournals.jbchem.a131952. PMID 204628.
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Restriction modification system: restriction enzyme
Basic Concept
  • Isoschizomer
  • Neoschizomer
  • Isocaudomer
Recognition sequence 4bp
  • TaqI
  • Sau3A
  • AluI*
  • HaeIII*
  • DpnII
  • NlaIII
  • HpaII
Recognition sequence 5bp
  • EcoRII
  • FokI
Recognition sequence 6bp
  • AaaI
  • BglII
  • PovII*
  • EcoRV*
  • EcoRI
  • BamHI
  • HindIII
  • XbaI
  • XhoI
  • SacI
  • PstI
  • NdeI
Recognition sequence 8bp
  • NotI
Lists
  • A
  • Ba–Bc
  • Bd–Bp
  • Bsa–Bso
  • Bsp–Bss
  • Bst–Bv
  • C-D
  • E-F
  • G-K
  • L-N
  • O-R
  • S
  • T-Z
* means cleavage produces blunt ends
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